Recombinant humaan BEND6-eiwit

BEND6, as known as BEN domain-containing protein 6, is a neural BEN-solo factor that shares many functional attributes with Drosophila Insensitive, a co-repressor for the Drosophila CSL factor. This protein binds the mammalian CSL protein CBF1 and antagonizes Notch-dependent target activation. In addition, its association with Notch- and CBF1-regulated enhancers is promoted by CBF1 and antagonized by activated Notch. In utero electroporation experiments showed that ectopic BEND6 inhibited Notch-mediated self-renewal of neocortical neural stem cells and promoted neurogenesis. Recombinant human BEND6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

BEN domain-containing protein 6, bA203B9.1, C6orf65

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.6 kDa (302aa) confirmed by MALDI-TOF

BEND6, BEN domain-containing protein 6, bA203B9.1, C6orf65, ATGP2441-10 µg, ATGP2441-20 µg, ATGP2441-50 µg, ATGP2441-100 µg, ATGP2441-250 µg, ATGP2441-500 µg, ATGP2441-1 mg, ATGP2441-10, ATGP2441-20, ATGP2441-50, ATGP2441-100, ATGP2441-250, ATGP2441-500, ATGP2441-1

Dai Q. et al. (2013) Development.. 140:1892-1902; ; Chu J. et al. (2004) J Biol Chem. 279:12337-12345.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Other

NP_689944

Q5SZJ8

Human

MQKIVQT DEITNTQAFR KGKRKRTETM DSENANSDMD KGQRDPYSGN AFLPGESSSE DEEPLAELSK EELCAKIKSL KEKLTNTRKE NSRLRQSLVM LQVLPQAVTQ FEELVGMAEA LLKGGGTMST SASTLWRATN NSSPDSFAST CSNSNSNSSS PVSLKPEEEH QTDEKQFQIE KWQIARCNKS KPQKFINDLM QVLYTNEYMA THSLTGAKSS TSRDKAVKPA MNQNEVQEII GVTKQLFPNT DDVSIRRMIG QKLNNCTKKP NLSKNLNSQD IK