Recombinant humaan BEST1-eiwit

BEST1 is a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Recombinant human BEST1 protein, fused to His-tag at N-terminus, was expressed in E. coli

Bestrophin-1 isoform 1, ARB, BEST, BMD, RP50, Tu15B, VMD2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36 kDa (317aa)

BEST1, Bestrophin-1 isoform 1, ARB, BEST, BMD, RP50, Tu15B, VMD2, ATGP2600-10 µg, ATGP2600-20 µg, ATGP2600-50 µg, ATGP2600-100 µg, ATGP2600-250 µg, ATGP2600-500 µg, ATGP2600-1 mg, ATGP2600-10, ATGP2600-20, ATGP2600-50, ATGP2600-100, ATGP2600-250, ATGP2600-500, ATGP2600-1

Sodi A, Passerini I, et al. (2012) . Mol Vis.18:2736-48.; ; Bitner H, Schatz P, et al. (2012) . Am J Ophthalmol. 154 (2) :403-412.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Neuroscience

NP_004174.1

O76090

Human

EQLINPF GEDDDDFETN WIVDRNLQVS LLAVDEMHQD LPRMEPDMYW NKPEPQPPYT AASAQFRRAS FMGSTFNISL NKEEMEFQPN QEDEEDAHAG IIGRFLGLQS HDHHPPRANS RTKLLWPKRE SLLHEGLPKN HKAAKQNVRG QEDNKAWKLK AVDAFKSAPL YQRPGYYSAP QTPLSPTPMF FPLEPSAPSK LHSVTGIDTK DKSLKTVSSG AKKSFELLSE SDGALMEHPE VSQVRRKTVE FNLTDMPEIP ENHLKEPLEQ SPTNIHTTLK DHMDPYWALE NRDEAHS