Recombinant humaan bèta-Galactosidase-1/GLB1-eiwit

GLB1, also known as beta-galactosidase, is a lysosomal B-galactosidase that hydrolyzes the terminal B-galactose form ganglioside and keratin sulfate. It plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. It seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, it associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers. Recombinant human GLB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

GLB1, EBP, ELNR1, MPS4B

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

74.6 kDa (662aa)

B-Galactosidase1, GLB1, ATGP3290-10 µg, ATGP3290-20 µg, ATGP3290-50 µg, ATGP3290-100 µg, ATGP3290-250 µg, ATGP3290-500 µg, ATGP3290-1 mg, ATGP3290-10, ATGP3290-20, ATGP3290-50, ATGP3290-100, ATGP3290-250, ATGP3290-500, ATGP3290-1

Hofer D., et al. (2009) Hum. Mutat 30:1214-1221.; ; Kaye E.M., et al. (1997) J. Child Neurol. 12:242-247.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_000395.2

P16278

Human