Recombinant humaan Beta-glucuronidase/GUSB-eiwit

GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH3.5

Beta-glucuronidase isoform 1, GUSB, BG, MPS7, Glucuronidase beta, Beta-G1

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 4000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylum-belliferyl-beta-D-glucosiduronic acid to 4-Methylumbelliferone per minute at 37C and pH3.5.

73.4 kDa (635aa)

GUSB, Beta-glucuronidase isoform 1, GUSB, BG, MPS7, ATGP3495-10 µg, ATGP3495-20 µg, ATGP3495-50 µg, ATGP3495-100 µg, ATGP3495-250 µg, ATGP3495-500 µg, ATGP3495-1 mg, ATGP3495-10, ATGP3495-20, ATGP3495-50, ATGP3495-100, ATGP3495-250, ATGP3495-500, ATGP3495-1

Shipley JM., et al. (1993) Am J Hum Genet. 52:517-526.; ; Bell CE Jr., et al. (1977) J Clin Invest. 59:97-105.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_000172.2

P08236

Human

Leu23