Recombinant humaan Betacelluline-eiwit

BTC (Betacellulin) is a member of the EGF family of growth factors and contains 1 EGF-like domain. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. The effects of BTC are probably mediated by the EGF receptor and other related receptors. Recombinant human BTC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Betacellulin, probetacellulin precursor

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

11.3kD (101aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Bcellulin, Betacellulin, probetacellulin precursor, ATGP1250-10 µg, ATGP1250-20 µg, ATGP1250-50 µg, ATGP1250-100 µg, ATGP1250-250 µg, ATGP1250-500 µg, ATGP1250-1 mg, ATGP1250-10, ATGP1250-20, ATGP1250-50, ATGP1250-100, ATGP1250-250, ATGP1250-500, ATGP1250-1

Gratao AA, et al. (2008) Biol Reprod.78 (1) :43-52.; ; Genetos DC, et al. (2010) Cell Tissue Res. 340 (1) :81-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 200 mM NaCl

Growth factors

NP_001720.1

P35070

Human

DGNSTRSPE TNGLLCGDPE ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI GARCERVDLF Y