Recombinant humaan Betacelluline/BTC-eiwit

BTC, also known as Betacellulin, is a member of epidermal growth factor receptor family and functions as a ligand for the epidermal growth factor receptor (EGFR) . As the role a EGFR, betacellulin is manifested by different form of muscles and tissues, it also has a great effect of nitrogen that is used for retinal pigment epithelial cells and vascular smooth muscle cells. While many studies attest a role for betacellulin in the differentiation of pancreatic β-cells, the last decade witnessed the association of betacellulin with many additional biological processes, ranging from reproduction to the control of neural stem cells. As a typical EGFR ligand, betacellulin is expressed by a variety of cell types and tissues, and the soluble growth factor is proteolytically cleaved from a larger membrane-anchored precursor. It stimulates the proliferation of retinal pigment epithelial and vascular smooth muscle cells but did not stimulate the growth of several other cell types, such as endothelial cells and fetal lung fibroblasts. Recombinant human Betacellulin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Human BTC stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.5 ng/mL.

Betacellulin, Probetacellulin, BTC, Betacellulin isoform 1

HEK293

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.5 ng/mL.

9.8kDa (86aa)

ATGP4016-10 µg, ATGP4016-20 µg, ATGP4016-50 µg, ATGP4016-100 µg, ATGP4016-250 µg, ATGP4016-500 µg, ATGP4016-1 mg, ATGP4016-10, ATGP4016-20, ATGP4016-50, ATGP4016-100, ATGP4016-250, ATGP4016-500, ATGP4016-1, ATGP4016-10 µg, ATGP4016-20 µg, ATGP4016-50 µg, ATGP4016-100 µg, ATGP4016-250 µg, ATGP4016-500 µg, ATGP4016-1 mg, ATGP4016-10, ATGP4016-20, ATGP4016-50, ATGP4016-100, ATGP4016-250, ATGP4016-500, ATGP4016-1

Saito T., et al, (2004) Endocrinology. 145 (9) :4232-4243.; ; Kim HS., et al, (2003) FASEB Journal. 17 (2) :318-320.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Signal Transduction

NP_001720

P35070

Human

Asp32