Recombinant humaan Biglycan eiwit

BGN, also known as biglycan, is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. Recombinant human BGN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Biglycan preproprotein, DSPG1, PG-S1, PGI, SLRR1A

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.5 kDa (352aa)

Biglycan, Biglycan preproprotein, DSPG1, PG-S1, PGI, SLRR1A, ATGP1988-10 µg, ATGP1988-20 µg, ATGP1988-50 µg, ATGP1988-100 µg, ATGP1988-250 µg, ATGP1988-500 µg, ATGP1988-1 mg, ATGP1988-10, ATGP1988-20, ATGP1988-50, ATGP1988-100, ATGP1988-250, ATGP1988-500, ATGP1988-1

Babelova A. et al. (2009) J Biol Chem. 284:24035-24048.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Signal Transduction

NP_001702

P21810

Human

MGSSHHHHHH SSGLVPRGSH MDEEASGADT SGVLDPDSVT PTYSAMCPFG CHCHLRVVQC SDLGLKSVPK EISPDTTLLD LQNNDISELR KDDFKGLQHL YALVLVNNKI SKIHEKAFSP LRKLQKLYIS KNHLVEIPPN LPSSLVELRI HDNRIRKVPK GVFSGLRNMN CIEMGGNPLE NSGFEPGAFD GLKLNYLRIS EAKLTGIPKD LPETLNELHL DHNKIQAIEL EDLLRYSKLY RLGLGHNQIR MIENGSLSFL PTLRELHLDN NKLARVPSGL PDLKLLQVVY LHSNNITKVG VNDFCPMGFG VKRAYYNGIS LFNNPVPYWE VQPATFRCVT DRLAIQFGNY KK