Recombinant humaan Biglycan eiwit

BGN, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP) . It plays an important role in the development and maintenance of many tissues. Core protein of this protein binds to the growth factors BMP-4 and influences its bioactivity. This protein is also involved in collagen fiber assembly. Recombinant human BGN protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human Biglycan in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 range ≤ 20 µg/mL.

Biglycan preproprotein, BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 range ≤ 20 µg/mL.

38.3 kDa (340aa)

Biglycan, Biglycan preproprotein, BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A, ATGP3776-10 µg, ATGP3776-20 µg, ATGP3776-50 µg, ATGP3776-100 µg, ATGP3776-250 µg, ATGP3776-500 µg, ATGP3776-1 mg, ATGP3776-10, ATGP3776-20, ATGP3776-50, ATGP3776-100, ATGP3776-250, ATGP3776-500, ATGP3776-1

Sun H., et al, (2016) Arch Gynecol Obstet. 293:429-438.; ; Gaspar R., et al, (2016) J Mol Cell Cardiol. 99:138-150.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001702.1

P21810

Human

Ala

DEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKK