Recombinant humaan Biliverdin Reductase A/BLVRA-eiwit

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) Recombinant BLVRA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein.

E.coli

Human

Non-Tagged

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

33.3 kDa (295aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Biliverdin Reductase A, BLVRA, Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein., ATGP0377-10 µg, ATGP0377-20 µg, ATGP0377-50 µg, ATGP0377-100 µg, ATGP0377-250 µg, ATGP0377-500 µg, ATGP0377-1 mg, ATGP0377-10, ATGP0377-20, ATGP0377-50, ATGP0377-100, ATGP0377-250, ATGP0377-500, ATGP0377-1

Kravets A., et al. (2004) J Biol Chem. 279 (19) :19916-23.; ; Franklin E., et al. (2007) Biochem J. 405 (1) :61-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

NP_000703

P53004

Human

MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK