Recombinant humaan BIM/BCL2L11-eiwit

BCL2L11 (Bcl-2-like protein 11) belongs to the Bcl-2 family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of BCL2L11 can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Recombinant human BCL2L11 protein, fused to His-tag at N-terminus, was expressed in E. coli.

BCL2-like 11, BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BimEL, BimL, BOD

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

18.5 kDa (162aa)

BCL2L11, BCL2-like 11, BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BimEL, BimL, BOD, ATGP1661-10 µg, ATGP1661-20 µg, ATGP1661-50 µg, ATGP1661-100 µg, ATGP1661-250 µg, ATGP1661-500 µg, ATGP1661-1 mg, ATGP1661-10, ATGP1661-20, ATGP1661-50, ATGP1661-100, ATGP1661-250, ATGP1661-500, ATGP1661-1

O'Connor L, et al. (1998) . EMBO J 17 (2) : 384-95.; ; Liu J.-W., et al. (2002) Cancer Res. 62:2976-2981

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol 5mM DTT, 300 mM NaCl

Apoptosis

NP_006529.1

O43521

Human

MAKQPS DVSSECDREG RQLQPAERPP QLRPGAPTSL QTEPQDRSPA PMSCDKSTQT PSPPCQAFNH YLSAMASMRQ AEPADMRPEI WIAQELRRIG DEFNAYYARR VFLNNYQAAE DHPRMVILRL LRYIVRLVWR MH