Recombinant humaan BIN1-eiwit

BIN1, also known as SH3P9, is a nucleocytoplasmic adaptor protein, one of which was initially identified as MYC-interacting protein with features of a tumor suppressor. This protein interacts with and inhibits the oncogenic activity of the myc oncoprotein that has a major role in many human cancers. The loss of Bin1 may contribute to growth deregulation in cancer cells in carcinoma of the breast, colon, lung, cervix, prostate and liver. Recombinant human BIN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myc box-dependent-interacting protein 1, AMPH2, AMPHL, MGC10367, SH3P9, Amphiphysin II, Myc box-dependent-interacting protein 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

50.4 kDa (459aa) confirmed by MALDI-TOF

BIN1, Myc box-dependent-interacting protein 1, AMPH2, AMPHL, MGC10367, SH3P9, Amphiphysin II, Myc box-dependent-interacting protein 1, ATGP0673-10 µg, ATGP0673-20 µg, ATGP0673-50 µg, ATGP0673-100 µg, ATGP0673-250 µg, ATGP0673-500 µg, ATGP0673-1 mg, ATGP0673-10, ATGP0673-20, ATGP0673-50, ATGP0673-100, ATGP0673-250, ATGP0673-500, ATGP0673-1

Sakamuro D., et al. (1996) Nat Genet. 14 (1) :69-77. ; ; Nanda SK., et al. (2006) Gastroenterology. 130 (3) :794-809.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Cancer

AAH04101.1

Q9BTH3

Human

MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLNECLQEVY EPDWPGRDEA NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY DSARHHYESL QTAKKKDEAK IAKAEEELIK AQKVFEEMNV DLQEELPSLW NSRVGFYVNT FQSIAGLEEN FHKEMSKLNQ NLNDVLVGLE KQHGSNTFTV KAQPSDNAPA KGNKSPSPPD GSPAATPEIR VNHEPEPAGG ATPGATLPKS PSQPAEASEV AGGTQPAAGA QEPGETAASE AASSSLPAVV VETFPATVNG TVEGGSGAGR LDLPPGFMFK VQAQHDYTAT DTDELQLRAG DVVLVIPFQN PEEQDEGWLM GVKESDWNQH KELEKCRGVF PENFTERVP