Recombinant humaan Biotinidase/BTD-eiwit

BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Biotinidase isoform 1, BTD, biotinidase

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

57.8 kDa (510aa)

Biotinidase, BTD, Biotinidase isoform 1, BTD, biotinidase, ATGP3461-10 µg, ATGP3461-20 µg, ATGP3461-50 µg, ATGP3461-100 µg, ATGP3461-250 µg, ATGP3461-500 µg, ATGP3461-1 mg, ATGP3461-10, ATGP3461-20, ATGP3461-50, ATGP3461-100, ATGP3461-250, ATGP3461-500, ATGP3461-1

Hymes J., et al. (2001) Hum Mutat. 18:375-381.; ; Cole H., et al. (1994) J Biol Chem. 269:6566-6570.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001268652

P43251

Human

Ala44

AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH