Recombinant humaan BLNK-eiwit

BLNK, also known as B-cell linker, is essential for normal B-cell development. This protein has been shown to associate with the effector proteins GRB2, Vav, NCK and PLC gamma following activation of the B cell receptor. It is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC gamma. Recombinant human BLNK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

B-cell linker, BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

52.6 kDa (476aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

BLNK, B-cell linker, BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65, ATGP0915-10 µg, ATGP0915-20 µg, ATGP0915-50 µg, ATGP0915-100 µg, ATGP0915-250 µg, ATGP0915-500 µg, ATGP0915-1 mg, ATGP0915-10, ATGP0915-20, ATGP0915-50, ATGP0915-100, ATGP0915-250, ATGP0915-500, ATGP0915-1

Kurosaki T, et al. (1997) Curr. Opin. Immunol., 9:309-318.; ; Fu C, et al. (1998) . Immunity. 9:93-103.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF

Signal Transduction

AAH18906.1

Q8WV28

Human

MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS