Recombinant humaan BMF-eiwit

Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Bcl2 modifying factor isoform 3, Bcl2 modifying factor, isoform 3, BMF, Bcl2 modifying factor, isoform 3 Bcl 2 modifying factor, FLJ00065.

E.coli

Human

T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

15.6 kDa (144aa) confirmed by MALDI-TOF

BMF, Bcl2 modifying factor isoform 3, Bcl2 modifying factor, isoform 3, BMF, Bcl2 modifying factor, isoform 3 Bcl 2 modifying factor, FLJ00065., ATGP0324-10 µg, ATGP0324-20 µg, ATGP0324-50 µg, ATGP0324-100 µg, ATGP0324-250 µg, ATGP0324-500 µg, ATGP0324-1 mg, ATGP0324-10, ATGP0324-20, ATGP0324-50, ATGP0324-100, ATGP0324-250, ATGP0324-500, ATGP0324-1

Morales AA., et al. (2004) . Leukemia. 18 (1) :41-7; ; Chen L., et al. (2005) . Mol Cell. 17 (3) :393-403;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol

Cancer

NP_001003943

Q96LC9

Human

MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP