Recombinant humaan BMP-14/GDF-5 eiwit

Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Growth differentiation factor 5, Bone morphogenetic protein 14 (BMP-14), Cartilage-derived morphogenetic protein 1 (CDMP-1), Lipopolysaccharide-associated protein 4 (LAP-4; LPS-associated protein 4), Radotermin, BMP14, CDMP1, LAP4, OS5, SYNS2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

15.8 kDa (141aa) confirmed by MALDI-TOF

BMP 14, BMP14, BMP-14, Bone morphogenetic protein 14, Cartilage derived morphogenetic protein 1, Cartilage-derived morphogenetic protein 1, CDMP 1, CDMP1, CDMP-1, GDF 5, GDF5, GDF-5, Growth differentiation factor 5, LAP 4, LAP4, LAP-4, Lipopolysaccharide associated protein 4, Lipopolysaccharide-associated protein 4, LPS associated protein 4, LPS-associated protein 4, OS5, Radotermin, SYNS2, ATGP0334-10 µg, ATGP0334-20 µg, ATGP0334-50 µg, ATGP0334-100 µg, ATGP0334-250 µg, ATGP0334-500 µg, ATGP0334-1 mg, ATGP0334-010, ATGP0334-020, ATGP0334-050, ATGP0334-100, ATGP0334-250, ATGP0334-500, ATGP0334-01M

Feng G., et al. (2008) . Growth Factor. 26 (3) :132-42; ; Sullivan AM., et al. (2005) . J Anat. 207 (3) :219-26.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol

Hormones & Cytokines

NP_000548

P43026

Human

MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R