Recombinant humaan BMP-2-eiwit

BMP-2, also known as Bone morphogenetic protein 2, is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility. Recombinant human BMP2 was expressed in E. coli and purified by conventional chromatography techniques.

Human BMP-2 stimulates alkaline phosphatase in the ATDC5 mouse chondrogenic cells. The ED50 range ≤ 100 ng/mL.

Bone morphogenetic protein 2, BMP-2A

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is ≤ 100 ng/mL.

13 kDa (115aa)

BMP2, Bone morphogenetic protein 2, BMP-2A, ATGP3940-10 µg, ATGP3940-20 µg, ATGP3940-50 µg, ATGP3940-100 µg, ATGP3940-250 µg, ATGP3940-500 µg, ATGP3940-1 mg, ATGP3940-10, ATGP3940-20, ATGP3940-50, ATGP3940-100, ATGP3940-250, ATGP3940-500, ATGP3940-1

Chen D., et al. (2004) . Growth factors. 22 (4) :233-41; ; Schliephake H., et al. (2005) . Clin Oral Implants Res. 17 (6) :666-72

Tanaka M, et al. Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration. (Materials (Basel) . 2017) {https://pubmed.ncbi.nlm.nih.gov/28772390/}; ; Tanaka M, et al. In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration. (Nanomaterials (Basel) . 2017) {https://pubmed.ncbi.nlm.nih.gov/28336879/}; ; Qi S, et al. Cellular Internalization-Induced Aggregation of Porous Silicon Nanoparticles for Ultrasound Imaging and Protein-Mediated Protection of Stem Cells. (Small. 2019) {https://pubmed.ncbi.nlm.nih.gov/30488562/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 10 mM Sodium acetate (pH 3.5)

Signal Transduction

NP_001191

P12643

Human

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR