Recombinant humaan BNPNAT1-eiwit

Glucosamine-phosphate N-acetyltransferase, also known GNPNAT1, belongs to the GNA1 subfamily of the larger acetyltransferase family of proteins. It is localized to the Golgi apparatus and the endosome. It is important for uDPGlcNAc biosynthesis pathway. GNPNAT1 catalyzes the synthesis of GlcNAc6P from AcCoA and GlcN6P, a step in the uDP-GlcNAc6P formation pathway. Recombinant human GNPNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glucosamine-phosphate N-acetyltransferase, GNPNAT, Gpnat1, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase, GNA1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.1 kDa (207aa) confirmed by MALDI-TOF

GNPNAT1, Glucosamine-phosphate N-acetyltransferase, GNPNAT, Gpnat1, ATGP0817-10 µg, ATGP0817-20 µg, ATGP0817-50 µg, ATGP0817-100 µg, ATGP0817-250 µg, ATGP0817-500 µg, ATGP0817-1 mg, ATGP0817-10, ATGP0817-20, ATGP0817-50, ATGP0817-100, ATGP0817-250, ATGP0817-500, ATGP0817-1

Jiang, H., et al. (2005) Plant Physiol. 138:232-242.; ; Mio T., et al. (2007) Microbiology. 146:1753-1758.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_932332

Q96EK6

Human

MGSSHHHHHH SSGLVPRGSH MGSMKPDETP MFDPSLLKEV DWSQNTATFS PAISPTHPGE GLVLRPLCTA DLNRGFFKVL GQLTETGVVS PEQFMKSFEH MKKSGDYYVT VVEDVTLGQI VATATLIIEH KFIHSCAKRG RVEDVVVSDE CRGKQLGKLL LSTLTLLSKK LNCYKITLEC LPQNVGFYKK FGYTVSEENY MCRRFLK