Recombinant humaan BOLA1-eiwit

BOLA1 belongs to the bolA/yrbA family. BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Recombinant human BOLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

BolA-like protein 1, CGI-143

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14 kDa (140aa) confirmed by MALDI-TOF

BOLA1, BolA-like protein 1, CGI-143, ATGP1911-10 µg, ATGP1911-20 µg, ATGP1911-50 µg, ATGP1911-100 µg, ATGP1911-250 µg, ATGP1911-500 µg, ATGP1911-1 mg, ATGP1911-10, ATGP1911-20, ATGP1911-50, ATGP1911-100, ATGP1911-250, ATGP1911-500, ATGP1911-1

Zhou YB, Cao JB, et al. (2008) . Mol Cell Biochem. 317 (1-2) :61-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Other

NP_057158

Q9Y3E2

Human

MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP