Recombinant humaan BPHL-eiwit

BPHL is a Serine hydrolase which Belongs to the AB hydrolase superfamily. This family catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. This protein is expressed at high levels in liver and kidney and at lower levels in heart, intestine and skeletal muscle. It plays a role in detoxification processes. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Recombinant human BPHL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Valacyclovir hydrolase, BPH-RP, MCNAA, VACVASE

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.1 kDa (275aa) confirmed by MALDI-TOF

BPHL, Valacyclovir hydrolase, BPH-RP, MCNAA, VACVASE, ATGP0870-10 µg, ATGP0870-20 µg, ATGP0870-50 µg, ATGP0870-100 µg, ATGP0870-250 µg, ATGP0870-500 µg, ATGP0870-1 mg, ATGP0870-10, ATGP0870-20, ATGP0870-50, ATGP0870-100, ATGP0870-250, ATGP0870-500, ATGP0870-1

Lai L., et al. (2008) J. Biol. Chem. 283:9318-9327.; ; Puente X.S., et al. (1995) J. Biol. Chem. 270:12926-12932;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Metabolism

NP_004323

Q86WA6

Human

MGSSHHHHHH SSGLVPRGSH MSVTSAKVAV NGVQLHYQQT GEGDHAVLLL PGMLGSGETD FGPQLKNLNK KLFTVVAWDP RGYGHSRPPD RDFPADFFER DAKDAVDLMK ALKFKKVSLL GWSDGGITAL IAAAKYPSYI HKMVIWGANA YVTDEDSMIY EGIRDVSKWS ERTRKPLEAL YGYDYFARTC EKWVDGIRQF KHLPDGNICR HLLPRVQCPA LIVHGEKDPL VPRFHADFIH KHVKGSRLHL MPEGKHNLHL RFADEFNKLA EDFLQ