Recombinant humaan BPNT1-eiwit

BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1, 4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and the conversion of PAP (3' (2') -phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate) . Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3' (2') 5'-bisphosphate nucleotidase 1, 3' (2'), 5'-bisphosphate nucleotidase 1, PIP, PAP-inositol-1,4-phosphatase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.5 kDa (344aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

BPNT1, 3' (2') 5'-bisphosphate nucleotidase 1, 3' (2'), 5'-bisphosphate nucleotidase 1, PIP, PAP-inositol-1,4-phosphatase, ATGP0883-10 µg, ATGP0883-20 µg, ATGP0883-50 µg, ATGP0883-100 µg, ATGP0883-250 µg, ATGP0883-500 µg, ATGP0883-1 mg, ATGP0883-10, ATGP0883-20, ATGP0883-50, ATGP0883-100, ATGP0883-250, ATGP0883-500, ATGP0883-1

Shaltiel G., et al. (2002) Bipolar Disord. 4 (5) :302-6. ; ; Agam G., et al. (2003) J Psychiatr Res. 37 (5) :433-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 5mM DTT, 0.1M NaCl.

Neuroscience

NP_006076

O95861

Human

MASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP