Recombinant humaan BRDG1/STAP1-eiwit

STAP1, also known as signal-transducing adaptor protein 1, appears to functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. Recombinant human STAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Signal-transducing adaptor protein 1, BRDG1, STAP-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

36.8 kDa (319aa) confirmed by MALDI-TOF

BRDG1, Signal-transducing adaptor protein 1, BRDG1, STAP-1, ATGP1552-10 µg, ATGP1552-20 µg, ATGP1552-50 µg, ATGP1552-100 µg, ATGP1552-250 µg, ATGP1552-500 µg, ATGP1552-1 mg, ATGP1552-10, ATGP1552-20, ATGP1552-50, ATGP1552-100, ATGP1552-250, ATGP1552-500, ATGP1552-1

Ohya K., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:11976-11981; ; Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_036240

Q9ULZ2

Human

MMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA