Recombinant humaan BRK1-eiwit

BRK1 involved in regulation of actin and microtubule organization. This protein is a part of a WAVE complex that activates the Arp2/3 complex. It belongs to the BRK1 family. Recombinant human BRK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein BRICK1, C3orf10, hHBrk1, HSPC300, MDS027, BRICK1 subunit of SCAR/WAVE actin nucleating complex, Haematopoietic stem/progenitor cell protein 300

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

11.1 kDa (98aa) confirmed by MALDI-TOF

BRK1, Protein BRICK1, C3orf10, hHBrk1, HSPC300, MDS027, ATGP2513-10 µg, ATGP2513-20 µg, ATGP2513-50 µg, ATGP2513-100 µg, ATGP2513-250 µg, ATGP2513-500 µg, ATGP2513-1 mg, ATGP2513-10, ATGP2513-20, ATGP2513-50, ATGP2513-100, ATGP2513-250, ATGP2513-500, ATGP2513-1

Eden S, Rohatgi R, et al. (2002) . Nature. 418 (6899) :790-3.; ; Cascon A, Escobar B, et al. (2007) . Hum Mutat. 28 (6) :613-21.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_060932

Q8WUW1

Human

MAGQEDP VQREIHQDWA NREYIEIITS SIKKIADFLN SFDMSCRSRL ATLNEKLTAL ERRIEYIEAR VTKGETLT