Recombinant humaan BSND-eiwit

BSND is an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this BSND have been associated with Bartter syndrome with sensorineural deafness. Recombinant human BSND protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Barttin, BART, DFNB73

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.7 kDa (290aa) confirmed by MALDI-TOF

BSND, Barttin, BART, DFNB73, ATGP2204-10 µg, ATGP2204-20 µg, ATGP2204-50 µg, ATGP2204-100 µg, ATGP2204-250 µg, ATGP2204-500 µg, ATGP2204-1 mg, ATGP2204-10, ATGP2204-20, ATGP2204-50, ATGP2204-100, ATGP2204-250, ATGP2204-500, ATGP2204-1

Park, C.W., et al. (2011) Clin. Nephrol. 75 (SuPPL 1), 69-74; ; Iqbal, H., et al. (2011) J. Biomed. Biotechnol. 2011, 304612;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_476517

Q8WZ55

Human

CQCYPKI TFVPADSDFQ GILSPKAMGL LENGLAAEMK SPSPQPPYVR LWEEAAYDQS LPDFSHIQMK VMSYSEDHRS LLAPEMGQPK LGTSDGGEGG PGDVQAWMEA AVVIHKGSDE SEGERRLTQS WPGPLACPQG PAPLASFQDD LDMDSSEGSS PNASPHDREE ACSPQQEPQG CRCPLDRFQD FALIDAPTLE DEPQEGQQWE IALPNNWQRY PRTKVEEKEA SDTGGEEPEK EEEDLYYGLP DGAGDLLPDK ELGFEPDTQG