Recombinant humaan BST-2/Tetherine-eiwit

BST2, also known as bone marrow stromal antigen 2, is involved in the growth and development of B-cells. BST2 is a human cellular protein which inhibits retrovirus infection by preventing the diffusion of virus particles after budding from infected cells. Initially discovered as an inhibitor to HIV-1 infection in the absence of Vpu, tetherin has also been shown to inhibit the release of other viruses such as the Lassa and Marburg virions. Also, this protein may play a role in B-cell activation in rheumatoid arthritis. Recombinant human BST2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Bone marrow stromal antigen 2, HM1.24 antigen, Tetherin, CD317

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

14.8 kDa (133aa) confirmed by MALDI-TOF

BST2, Tetherin, Bone marrow stromal antigen 2, HM1.24 antigen, Tetherin, CD317, ATGP1117-10 µg, ATGP1117-20 µg, ATGP1117-50 µg, ATGP1117-100 µg, ATGP1117-250 µg, ATGP1117-500 µg, ATGP1117-1 mg, ATGP1117-10, ATGP1117-20, ATGP1117-50, ATGP1117-100, ATGP1117-250, ATGP1117-500, ATGP1117-1

Van Damme N., et al. (2008) Cell Host Microbe. 3:245-252; ; Schubert HL., et al. (2010) Proc Natl Acad Sci u S A. 107 (42) :17951-6

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Immunology

NP_004326

Q10589

Human

MGSSHHHHHH SSGLVPRGSH MSEACRDGLR AVMECRNVTH LLQQELTEAQ KGFQDVEAQA ATCNHTVMAL MASLDAEKAQ GQKKVEELEG EITTLNHKLQ DASAEVERLR RENQVLSVRI ADKKYYPSSQ DSS