Recombinant humaan BTLA-eiwit

BTLA, also known as B- and T-lymphocyte attenuator isoform 1, is an inhibitory molecule which belongs to the Ig superfamily. It is a type 1 transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. This protein is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1) . Also, it is a ligand for tumor necrosis factor (receptor) superfamily, member 14 (TNFRSF14), also known as herpes virus entry mediator (HVEM) . BTLA-HVEM complexes negatively regulate T-cell immune responses. Recombinant human BTLA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

B- and T-lymphocyte attenuator isoform 1, BTLA, BTLA1, CD272

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

15.7 kDa (136aa)

BTLA, B- and T-lymphocyte attenuator isoform 1, BTLA, BTLA1, CD272, ATGP3717-10 µg, ATGP3717-20 µg, ATGP3717-50 µg, ATGP3717-100 µg, ATGP3717-250 µg, ATGP3717-500 µg, ATGP3717-1 mg, ATGP3717-10, ATGP3717-20, ATGP3717-50, ATGP3717-100, ATGP3717-250, ATGP3717-500, ATGP3717-1

Watanabe N., et al, (2003) Nat. Immunol. 4:670-679. ; ; Gavrieli M., et al, (2003) Biochem. Biophys. Res. Commun. 312:1236-1243.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_861445.3

Q7Z6A9

Human

Ala

KESCDVQ LYIKRQSEHS ILAGDPFELE CPVKYCANRP HVTWCKLNGT TCVKLEDRQT SWKEEKNISF FILHFEPVLP NDNGSYRCSA NFQSNLIESH STTLYVTDVK SASERPSKDE MASRPWLLYS