Recombinant humaan Bub3-eiwit

Mitotic checkpoint protein BuB3, also known as BuB3, is a conserved component of the mitotic spindle assembly complex (MCC) . The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BuB3 protein. BuB3 is essential for the kinetochore localization of BuB1 and BuBR1. BuB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BuB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BuB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Mitotic checkpoint protein BuB3, BuB3L, hBuB3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.5 kDa (351aa)

Bub3, Mitotic checkpoint protein BuB3, BuB3L, hBuB3, ATGP1929-10 µg, ATGP1929-20 µg, ATGP1929-50 µg, ATGP1929-100 µg, ATGP1929-250 µg, ATGP1929-500 µg, ATGP1929-1 mg, ATGP1929-10, ATGP1929-20, ATGP1929-50, ATGP1929-100, ATGP1929-250, ATGP1929-500, ATGP1929-1

Martinez Exposito M J., et al. (1999) Proc Natl Acad Sci uSA. 96:8493-8498.; ; Warren C D., et al. (2002) Mol Biol Cell. 13:3029-3041.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cell Cycle

NP_004716

O43684

Human

MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T