Recombinant humaan BUD31 eiwit

BuD31 is a nuclear protein that belongs to the BuD31 (G10) family. It contains a putative nuclear translocation sequence, an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a putative Zinc-finger structure. The structure of BuD31 suggests that it may be a nuclear regulator of transcription. Recombinant human BuD31 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein BuD31 homolog, BuD31 homolog (S. cerevisiae), Cwc14, EDG-2, EDG2, fSAP17, G10, YCR063W

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

19.4 kDa (167aa) confirmed by MALDI-TOF

BUD31, protein BuD31 homolog, BuD31 homolog (S. cerevisiae), Cwc14, EDG-2, EDG2, fSAP17, G10, YCR063W, ATGP2135-10 µg, ATGP2135-20 µg, ATGP2135-50 µg, ATGP2135-100 µg, ATGP2135-250 µg, ATGP2135-500 µg, ATGP2135-1 mg, ATGP2135-10, ATGP2135-20, ATGP2135-50, ATGP2135-100, ATGP2135-250, ATGP2135-500, ATGP2135-1

Singh J, Kumar D, Ramakrishnan N., et al. 2005 Dec; ;71 (12) :8752-63.; ; Hla T., et al. (1995) Biochim Biophys Acta. 25; ;1260 (2) :227-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_003901

P41223

Human

MGSSHHHHHH SSGLVPRGSH MGSMPKVKRS RKAPPDGWEL IEPTLDELDQ KMREAETEPH EGKRKVESLW PIFRIHHQKT RYIFDLFYKR KAISRELYEY CIKEGYADKN LIAKWKKQGY ENLCCLRCIQ TRDTNFGTNC ICRVPKSKLE VGRIIECTHC GCRGCSG