Recombinant humaan c-Jun eiwit

The human protooncogene c-Jun is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. c-Jun and c-Fos form a complex in the nucleus. AP1 is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21, p19 and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. Recombinant human c-Jun protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcription factor AP-1, AP-1, AP1, c-Jun, jun proto-oncogene

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.3 kDa (261aa) confirmed by MALDI-TOF

C-Jun, Transcription factor AP-1, AP-1, AP1, c-Jun, jun proto-oncogene, ATGP0960-10 µg, ATGP0960-20 µg, ATGP0960-50 µg, ATGP0960-100 µg, ATGP0960-250 µg, ATGP0960-500 µg, ATGP0960-1 mg, ATGP0960-10, ATGP0960-20, ATGP0960-50, ATGP0960-100, ATGP0960-250, ATGP0960-500, ATGP0960-1

Bohmann D., et al. (1987) Science. 238 (4832) :1386-92. ; ; Bannister AJ., et al. (1993) Nucleic Acids Res. 21 (5) :1289-95.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Cancer

NP_002219

P05412

Human

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