Recombinant humaan c-kit Ligand/SCF-eiwit

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤10 ng/mL.

Kit ligand, KITLG, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, steel factor, SF, Kitl, KL-1, Familial progressive hyperpigmentation 2, FPH2, SLF, DFNA69

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤10 ng/mL.

18.5 kDa (165aa) confirmed by MALDI-TOF

SCF, ATGP3619-10 µg, ATGP3619-20 µg, ATGP3619-50 µg, ATGP3619-100 µg, ATGP3619-250 µg, ATGP3619-500 µg, ATGP3619-1 mg, ATGP3619-10, ATGP3619-20, ATGP3619-50, ATGP3619-100, ATGP3619-250, ATGP3619-500, ATGP3619-1

Zhang Z., et al. (2000) . Proc. Natl. Acad. Sci. U.S.A. 97, 7732.; ; Okada S , et al. (1992) . Nippon Rinsho , 50, 1872.

Lee SH, et al. Involvement of MITF-A, an alternative isoform of mi transcription factor, on the expression of tryptase gene in human mast cells. (Exp Mol Med. 2010) {https://pubmed.ncbi.nlm.nih.gov/20513998/}; ; Takahashi M, et al. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells. (Leukemia. 2014) {https://pubmed.ncbi.nlm.nih.gov/24189293/}; ; Yamada T, et al. CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. (Arch Dermatol Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/30483878/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Adhesion & Movement

NP_000890

P21583

Human

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA