Recombinant humaan C1GalT1/C1GalT1C1-eiwit

C1GALT1 generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from uDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis. Recombinant human C1GALT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Core 1 synthase glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, C1GALT, T-synthase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

41.4 kDa (357aa) confirmed by MALDI-TOF

C1GalT1, C1GalT1C1, Core 1 synthase glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, C1GALT, T-synthase, ATGP2181-10 µg, ATGP2181-20 µg, ATGP2181-50 µg, ATGP2181-100 µg, ATGP2181-250 µg, ATGP2181-500 µg, ATGP2181-1 mg, ATGP2181-10, ATGP2181-20, ATGP2181-50, ATGP2181-100, ATGP2181-250, ATGP2181-500, ATGP2181-1

Ju T., et al. (2002) J. Biol. Chem. 277:178-186; ; Zhu L., et al. (2009) Kidney Int. 76:190-198

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_064541

Q9NS00

Human

LLGEKVD TQPNVLHNDP HARHSDDNGQ NHLEGQMNFN ADSSQHKDEN TDIAENLYQK VRILCWVMTG PQNLEKKAKH VKATWAQRCN KVLFMSSEEN KDFPAVGLKT KEGRDQLYWK TIKAFQYVHE HYLEDADWFL KADDDTYVIL DNLRWLLSKY DPEEPIYFGR RFKPYVKQGY MSGGAGYVLS KEALKRFVDA FKTDKCTHSS SIEDLALGRC MEIMNVEAGD SRDTIGKETF HPFVPEHHLI KGYLPRTFWY WNYNYYPPVE GPGCCSDLAV SFHYVDSTTM YELEYLVYHL RPYGYLYRYQ PTLPERILKE ISQANKNEDT KVKLGNP