Recombinant humaan C4BPB-eiwit

C4BPB is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain of this protein assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. Recombinant human C4BPB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Complement component 4 binding protein beta, C4BP, C4b-binding protein beta chain

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29kDa (260aa) confirmed by MALDI-TOF

C4BPB, ATGP1664-10 µg, ATGP1664-20 µg, ATGP1664-50 µg, ATGP1664-100 µg, ATGP1664-250 µg, ATGP1664-500 µg, ATGP1664-1 mg, ATGP1664-10, ATGP1664-20, ATGP1664-50, ATGP1664-100, ATGP1664-250, ATGP1664-500, ATGP1664-1

Carlsson, S.et al. (2010) J. Biol. Chem. 285 (42), 32038-32046; ; Buil, A., et al. (2010) Blood 115 (23), 4644-4650

Sugimoto M, et al. Increased expression of Protein S in eyes with diabetic retinopathy and diabetic macular edema. (Sci Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/34001977/}; ; Urawa M, et al. Protein S is protective in pulmonary fibrosis. (J Thromb Haemost. 2016) {https://pubmed.ncbi.nlm.nih.gov/27172994/}; ; Yasuma T, et al. Amelioration of Diabetes by Protein S. (Diabetes. 2016) {https://pubmed.ncbi.nlm.nih.gov/27207541/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Immunology

NP_000707

P20851

Human

SDAEH CPELPPVDNS IFVAKEVEGQ ILGTYVCIKG YHLVGKKTLF CNASKEWDNT TTECRLGHCP DPVLVNGEFS SSGPVNVSDK ITFMCNDHYI LKGSNRSQCL EDHTWAPPFP ICKSRDCDPP GNPVHGYFEG NNFTLGSTIS YYCEDRYYLV GVQEQQCVDG EWSSALPVCK LIQEAPKPEC EKALLAFQES KNLCEAMENF MQQLKESGMT MEELKYSLEL KKAELKAKLL