Recombinant humaan C8G-eiwit

C8gamma, also known as C8G, is one of three polypeptides (along with C8 alpha and C8 beta) that constitutes C8, a component of the complement system. C8 participates in the formation of Membrane Attack Complex (MAC) . Patients with deficiency in C8 are vulnerable to certain bacteria infection. C8gamma is a secreted protein that is able to bind retinol and belongs to the lipocalin family and calycin superfamily. It is synthesised in the liver, monocytes and fibroblast and functions to clear pathogens from an infected host. Recombinant human C8G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Complement component 8 gamma polypeptide, complement component 8, gamma polypeptide, C8C

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

22.6 kDa (203aa) confirmed by MALDI-TOF

C8G, ATGP1235-10 µg, ATGP1235-20 µg, ATGP1235-50 µg, ATGP1235-100 µg, ATGP1235-250 µg, ATGP1235-500 µg, ATGP1235-1 mg, ATGP1235-10, ATGP1235-20, ATGP1235-50, ATGP1235-100, ATGP1235-250, ATGP1235-500, ATGP1235-1

Chiswell B., et al. (2007) Biochim Biophys Acta. 1774 (5) :637-44.; ; Schreck SF., et al. (2000) Biochim Biophys Acta. 1482 (1-2) :199-208.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Immunology

AAI13625

P07360

Human

QKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVHVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR