Recombinant humaan CACYBP-eiwit

CACYBP is primarily a nuclear protein that contains one CS domain and one SGS domain. It is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely serves as a molecular bridge in ubiquitin E3 complexes. It also participates in the ubiquitin-mediated degradation of beta-catenin. CACYBP is thought to be a potential inhibitor of cell growth and invasion in the gastric cancer cell through its effects on beta-catenin protein expression and transcriptional activation of TCF/LEF. Recombinant human CACYBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.4 kDa (205aa) confirmed by MALDI-TOF

CACYBP, Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein., ATGP0566-10 µg, ATGP0566-20 µg, ATGP0566-50 µg, ATGP0566-100 µg, ATGP0566-250 µg, ATGP0566-500 µg, ATGP0566-1 mg, ATGP0566-10, ATGP0566-20, ATGP0566-50, ATGP0566-100, ATGP0566-250, ATGP0566-500, ATGP0566-1

Matsuzawa SI., et al. (2001) Mol Cell. 7 (5) :915-26. ; ; Filipek A., et al. (2002) J Biol Chem. 277 (23) :21103-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Ubiquitination

NP_001007215

Q9HB71

Human

MQQKSQKKAE LLDNEKPAAV VAPITTGYTV KISNYGWDQS DKFVKIYITL TGVHQVPTEN VQVHFTERSF DLLVKNLNGK SYSMIVNNLL KPISVEGSSK KVKTDTVLIL CRKKVENTRW DYLTQVEKEC KEKEKPSYDT ETDPSEGLMN VLKKIYEDGD DDMKRTINKA WVESREKQAK GDTEF