Recombinant humaan Calcineurin B-eiwit

PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB) . Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Recombinant human PPP3R1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase 3 regulatory subunit B alpha, Calcineurin subunit B type 1, CALNB1, CNB, CNB1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.5 kDa (190aa) confirmed by MALDI-TOF

Calcineurin B, Protein phosphatase 3 regulatory subunit B alpha, Calcineurin subunit B type 1, CALNB1, CNB, CNB1, ATGP0901-10 µg, ATGP0901-20 µg, ATGP0901-50 µg, ATGP0901-100 µg, ATGP0901-250 µg, ATGP0901-500 µg, ATGP0901-1 mg, ATGP0901-10, ATGP0901-20, ATGP0901-50, ATGP0901-100, ATGP0901-250, ATGP0901-500, ATGP0901-1

Wang MG., et al. (1996) Cytogenet Cell Genet. 72 (2-3) :236-41.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Signal Transduction

NP_000936

P63098

Human

MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV