Recombinant humaan Calcineurine A/PPP3CA-eiwit

PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2. Recombinant human PPP3CA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CNA alpha, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CALNA, CALN, CNA, CNA1, PPP2B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

60 kDa (534aa)

PPP3CA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha, CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B, ATGP0861-10 µg, ATGP0861-20 µg, ATGP0861-50 µg, ATGP0861-100 µg, ATGP0861-250 µg, ATGP0861-500 µg, ATGP0861-1 mg, ATGP0861-10, ATGP0861-20, ATGP0861-50, ATGP0861-100, ATGP0861-250, ATGP0861-500, ATGP0861-1

Ostenfeld MS., et al. (2010) Oncogene. 29 (7) :1073-84.; ; Rodriguez A., et al. (2009) Mol Cell. 33 (5) :616-26.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol

Signal Transduction

NP_001124163

Q08209

Human

MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ