Recombinant humaan Calcineurine B2/PPP3R2-eiwit

PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase 3 regulatory subunit B beta, Protein phosphatase 2B regulatory subunit 2, Calcineurin subunit B type 2 19kDa, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, PPP3R1-like, PPP3RL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

22 kDa (193aa) confirmed by MALDI-TOF

PPP3R2, Protein phosphatase 3 regulatory subunit B type 2, Protein phosphatase 3, regulatory subunit B type 2, PPP3RL, ATGP0989-10 µg, ATGP0989-20 µg, ATGP0989-50 µg, ATGP0989-100 µg, ATGP0989-250 µg, ATGP0989-500 µg, ATGP0989-1 mg, ATGP0989-10, ATGP0989-20, ATGP0989-50, ATGP0989-100, ATGP0989-250, ATGP0989-500, ATGP0989-1

Ueki K., et al. (1992) Biochem Biophys Res Commun. 187 (1) :537-43. ; ; Liu L., et al. (2005) Mol Biol Rep. 32 (1) :41-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Signal Transduction

NP_671709.1

Q96LZ3

Human

MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV