Recombinant humaan calcium geactiveerd Nucleotidase 1/CANT1-eiwit

CANT1 (calcium-activated nucleotidase 1) belongs to the apyrase family. This protein is calcium-dependent nucleotidase with a preference for uDP. The order of activity with different substrates is uDP > GDP > uTP > GTP. Also, it has very low activity towards ADP and even lower activity towards ATP. And it does not hydrolyze AMP and GMP. The specific function of CANT1 is as yet unknown, but its substrates are involved in several major signaling functions, including Ca2+ release, through activation of pyrimidinergic signaling. Recombinant human CANT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Soluble calcium-activated nucleotidase 1, DBQD, SCAN-1, SCAN1, SHAPY

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40.5 kDa (364aa) confirmed by MALDI-TOF

Calcium Activated Nucleotidase1, CANT1, Soluble calcium-activated nucleotidase 1, DBQD, SCAN-1, SCAN1, SHAPY, ATGP1338-10 µg, ATGP1338-20 µg, ATGP1338-50 µg, ATGP1338-100 µg, ATGP1338-250 µg, ATGP1338-500 µg, ATGP1338-1 mg, ATGP1338-10, ATGP1338-20, ATGP1338-50, ATGP1338-100, ATGP1338-250, ATGP1338-500, ATGP1338-1

Yang M., et al. (2004) Biochemistry 43:9185-9194; ; Huber C, et al. (2009) Am J Hum Genet. 85 (5) :706-10.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Signal Transduction

NP_620148

Q8WVQ1

Human

MGSSHHHHHH SSGLVPRGSH MGSHMRPAPG RPPTHNAHNW RLGQAPANWY NDTYPLSPPQ RTPAGIRYRI AVIADLDTES RAQEENTWFS YLKKGYLTLS DSGDKVAVEW DKDHGVLESH LAEKGRGMEL SDLIVFNGKL YSVDDRTGVV YQIEGSKAVP WVILSDGDGT VEKGFKAEWL AVKDERLYVG GLGKEWTTTT GDVVNENPEW VKVVGYKGSV DHENWVSNYN ALRAAAGIQP PGYLIHESAC WSDTLQRWFF LPRRASQERY SEKDDERKGA NLLLSASPDF GDIAVSHVGA VVPTHGFSSF KFIPNTDDQI IVALKSEEDS GRVASYIMAF TLDGRFLLPE TKIGSVKYEG IEFI