Recombinant humaan Calponine 1-eiwit

CNN1, also known as calponin-1, belongs to the calponin family. CNN1 is thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Recombinant human CNN1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Calponin 1, CNN1, HEL-S-14, Sm-Calp, SMCC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.2 kDa (305aa) confirmed by MALDI-TOF

SMCC, Sm-Calp, Sm Calp, HEL-S-14, HEL S 14, CNN-1, CNN1, CNN 1, Calponin-1, Calponin1, Calponin 1, ATGP1584-10 µg, ATGP1584-20 µg, ATGP1584-50 µg, ATGP1584-100 µg, ATGP1584-250 µg, ATGP1584-500 µg, ATGP1584-1 mg, ATGP1584-010, ATGP1584-020, ATGP1584-050, ATGP1584-100, ATGP1584-250, ATGP1584-500, ATGP1584-01M

Maguchi M., et al. (1995) Biochem. Biophys. Res. Commun. 217:238-244; ; Miano J.M., et al. (1997) Gene. 197:215-224

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT

Actin & Myosin Related

NP_001290

P51911

Human

MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSALEH HHHHH