Recombinant humaan Calreticuline-eiwit

Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, Calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Recombinant human Calreticulin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CC1qR, CRT, FLJ26680, RO, SSA, Autoantigen RO, CALR, CALR protein, Calregulin, Calreticulin, CRP55, CRTC, ERp60, FLJ26680, grp60, HACBP, Sicca syndrome antigen A.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

48.7 kDa (421aa) confirmed by MALDI-TOF

Calreticulin, cC1qR, CRT, FLJ26680, RO, SSA, Autoantigen RO, CALR, CALR protein, Calregulin, Calreticulin, CRP55, CRTC, ERp60, FLJ26680, grp60, HACBP, Sicca syndrome antigen A., ATGP0542-10 µg, ATGP0542-20 µg, ATGP0542-50 µg, ATGP0542-100 µg, ATGP0542-250 µg, ATGP0542-500 µg, ATGP0542-1 mg, ATGP0542-10, ATGP0542-20, ATGP0542-50, ATGP0542-100, ATGP0542-250, ATGP0542-500, ATGP0542-1

Kropp LE., et al. (2010) J Immunol. 184 (10) :5619-27. ; ; Singh P., et al. (2010) J Biochem. 147 (5) :625-32.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol

Heat Shock Proteins

NP_004334.1

P27797

Human

EPAVYFKEQ FLDGDGWTSR WIESKHKSDF GKFVLSSGKF YGDEEKDKGL QTSQDARFYA LSASFEPFSN KGQTLVVQFT VKHEQNIDCG GGYVKLFPNS LDQTDMHGDS EYNIMFGPDI CGPGTKKVHV IFNYKGKNVL INKDIRCKDD EFTHLYTLIV RPDNTYEVKI DNSQVESGSL EDDWDFLPPK KIKDPDASKP EDWDERAKID DPTDSKPEDW DKPEHIPDPD AKKPEDWDEE MDGEWEPPVI QNPEYKGEWK PRQIDNPDYK GTWIHPEIDN PEYSPDPSIY AYDNFGVLGL DLWQVKSGTI FDNFLITNDE AYAEEFGNET WGVTKAAEKQ MKDKQDEEQR LKEEEEDKKR KEEEEAEDKE DDEDKDEDEE DEEDKEEDEE EDVPGQAKDE L