Recombinant humaan Calsequestrin 2/CASQ2-eiwit

Calsequestrin2 is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. It also helps sarcoplasmic reticulum store an amazing amount of calcium ions. Recombinant Calsequestrin2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Cardiac muscle calsequestrin 2, PDIB2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

48.4 kDa (417aa) confirmed by MALDI-TOF

Calsequestrin2, PDIB2, CASQ2, Calsequestrin 2 (cardiac muscle), Calsequestrin 2 fast twitch cardiac muscle, Calsequestrin cardiac muscle isoform, Calsequestrin fast twitch cardiac muscle, Calsequestrin2, Cardiac calsequestrin 2, CASQ 2, ESTM52, FLJ26321, FLJ93514, PDIB2., ATGP0502-10 µg, ATGP0502-20 µg, ATGP0502-50 µg, ATGP0502-100 µg, ATGP0502-250 µg, ATGP0502-500 µg, ATGP0502-1 mg, ATGP0502-10, ATGP0502-20, ATGP0502-50, ATGP0502-100, ATGP0502-250, ATGP0502-500, ATGP0502-1

Wang S, et al. (1998) Nat. Struct. Biol.. 5 (6) :476-83.; ; Lahat H, et al. (2001) Am J Hum Genet. 69 (6) :1378-84.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_001223

O14958

Human

GSMEEG LNFPTYDGKD RVVSLSEKNF KQVLKKYDLL CLYYHEPVSS DKVTQKQFQL KEIVLELVAQ VLEHKAIGFV MVDAKKEAKL AKKLGFDEEG SLYILKGDRT IEFDGEFAAD VLVEFLLDLI EDPVEIISSK LEVQAFERIE DYIKLIGFFK SEDSEYYKAF EEAAEHFQPY IKFFATFDKG VAKKLSLKMN EVDFYEPFMD EPIAIPNKPY TEEELVEFVK EHQRPTLRRL RPEEMFETWE DDLNGIHIVA FAEKSDPDGY EFLEILKQVA RDNTDNPDLS ILWIDPDDFP LLVAYWEKTF KIDLFRPQIG VVNVTDADSV WMEIPDDDDL PTAEELEDWI EDVLSGKINT EDDDEDDDDD DNSDEEDNDD SDDDDDE