Recombinant humaan CAMK2N1-eiwit

CAMK2N1 belongs to the CAMK2N family. This protein interacts with CAMK2B; the presence of Ca2+/calmodulin increases the interaction but is not essential. It also interacts with CAMK2A; this interaction requires CAMK2A activation by Ca2+. CAMK2N1 is potent and specific inhibitor of CaM-kinase II (CAMK2) . Recombinant human CAMK2N1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calcium/calmodulin-dependent protein kinase II inhibitor 1, CaMKII inhibitory protein alpha, CaMKIIN-alpha

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

10.9 kDa (101aa) confirmed by MALDI-TOF

Calcium calmodulin dependent protein kinase 2 inhibitor 1, Calcium calmodulin dependent protein kinase II inhibitor 1, Calcium/calmodulin dependent protein kinase II inhibitor 1, Calcium/calmodulin-dependent protein kinase 2 inhibitor 1, Calcium/calmodulin-dependent protein kinase II, Calcium/calmodulin-dependent protein kinase II inhibitor 1, CAMK2N1, CaMKII inhibitory protein alpha, CaMKIIN alpha, CaMKIIN-alpha, PRO1489, RP11 401M16.1, RP11-401M16.1, ATGP2567-10 µg, ATGP2567-20 µg, ATGP2567-50 µg, ATGP2567-100 µg, ATGP2567-250 µg, ATGP2567-500 µg, ATGP2567-1 mg, ATGP2567-010, ATGP2567-020, ATGP2567-050, ATGP2567-100, ATGP2567-250, ATGP2567-500, ATGP2567-01M

Wang, C., et al. (2008) . Biol. Chem. 283 (17), 11565-11574; ; Meng, F., et al. (2003) Brain Res. 967 (1-2), 161-169

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_061054

Q7Z7J9

Human

MGSSHHHHHH SSGLVPRGSH MGSMSEVLPY GDEKLSPYGD GGDVGQIFSC RLQDTNNFFG AGQNKRPPKL GQIGRSKRVV IEDDRIDDVL KNMTDKAPPG V