Recombinant humaan CAMLG-eiwit

The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. CAMLG functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. Recombinant human CAMLG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calcium signal-modulating cyclophilin ligand, CAML

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.2 kDa (212aa) confirmed by MALDI-TOF

CAMLG, Calcium signal-modulating cyclophilin ligand, CAML, ATGP2226-10 µg, ATGP2226-20 µg, ATGP2226-50 µg, ATGP2226-100 µg, ATGP2226-250 µg, ATGP2226-500 µg, ATGP2226-1 mg, ATGP2226-10, ATGP2226-20, ATGP2226-50, ATGP2226-100, ATGP2226-250, ATGP2226-500, ATGP2226-1

Bram RJ., et al. (1996) . Genomics 31 (2) : 257-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Signal Transduction

NP_001736.1

P49069

Human

MESMAVA TDGGERPGVP AGSGLSASQR RAELRRRKLL MNSEQRINRI MGFHRPGSGA EEESQTKSKQ QDSDKLNSLS VPSVSKRVVL GDSVSTGTTD QQGGVAEVKG TQLGDKLDSF IKPPECSSDV NLELRQRNRG DLTADSVQRG SRHGLEQYLS RFEEAMKLRK QLISEKPSQE DGNTTEEFDS FR