Recombinant humaan CAPNS1-eiwit

Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Recombinant human CAPNS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.8 kDa (208aa) confirmed by MALDI-TOF

CAPNS1, Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, ATGP2681-10 µg, ATGP2681-20 µg, ATGP2681-50 µg, ATGP2681-100 µg, ATGP2681-250 µg, ATGP2681-500 µg, ATGP2681-1 mg, ATGP2681-10, ATGP2681-20, ATGP2681-50, ATGP2681-100, ATGP2681-250, ATGP2681-500, ATGP2681-1

Ohno S., Emori Y, et al. (1986) Nucleic Acids Res. 14:5559-5559

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_001740

P04632

Human

MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS