Recombinant humaan CAPSL-eiwit

CAPSL, also known as Calcyphosine-like protein, is a calcium-binding protein containing two calcium-binding motifs (EF-hands) . These conserved domains are found in a superfamily of calcium sensors and calcium signal modulators. Furthermore, the CAPSL gene is in the same linkage disequilibrium (LD) block as the IL7R gene. It was reported association between the CAPSL-IL7R locus and type 1 diabetes. Recombinant human CAPSL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calcyphosine-like

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.3 kDa (228aa) confirmed by MALDI-TOF

CAPSL, Calcyphosine-like, ATGP0979-10 µg, ATGP0979-20 µg, ATGP0979-50 µg, ATGP0979-100 µg, ATGP0979-250 µg, ATGP0979-500 µg, ATGP0979-1 mg, ATGP0979-10, ATGP0979-20, ATGP0979-50, ATGP0979-100, ATGP0979-250, ATGP0979-500, ATGP0979-1

Liu S., et al. (2009) Hum Mol Genet. 18 (2) :358-65.; ; Santiago JL., et al. (2008) Diabetologia. 51 (9) :1653-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Signal Transduction

EAW55926

Q8WWF8

Human

MGSSHHHHHH SSGLVPRGSH MAGTARHDRE MAIQAKKKLT TATDPIERLR LQCLARGSAG IKGLGRVFRI MDDDNNRTLD FKEFMKGLND YAVVMEKEEV EELFQRFDKD GNGTIDFNEF LLTLRPPMSR ARKEVIMQAF RKLDKTGDGV ITIEDLREVY NAKHHPKYQN GEWSEEQVFR KFLDNFDSPY DKDGLVTPEE FMNYYAGVSA SIDTDVYFII MMRTAWKL