Recombinant humaan Carboxypeptide E/CPE-eiwit

CPE is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but constitutively secreted proteins. Mutations in CPE are implicated in type II diabetes. Recombinant human CPE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Carboxypeptidase E, Carboxypeptidase H, CPH

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

51.4 kDa (457aa)

Carboxypeptidase E, CPE, Carboxypeptidase E, Carboxypeptidase H, CPH, ATGP1953-10 µg, ATGP1953-20 µg, ATGP1953-50 µg, ATGP1953-100 µg, ATGP1953-250 µg, ATGP1953-500 µg, ATGP1953-1 mg, ATGP1953-10, ATGP1953-20, ATGP1953-50, ATGP1953-100, ATGP1953-250, ATGP1953-500, ATGP1953-1

Chu, K.Y., et al. (2011) Islets 3 (4), 155-165; ; Lee, T.K., et al. (2011) J. Clin. Invest. 121 (3), 880-892

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_001864

P16870

Human

MGSSHHHHHH SSGLVPRGSH MGSLQQEDGI SFEYHRYPEL REALVSVWLQ CTAISRIYTV GRSFEGRELL VIELSDNPGV HEPGEPEFKY IGNMHGNEAV GRELLIFLAQ YLCNEYQKGN ETIVNLIHST RIHIMPSLNP DGFEKAASQP GELKDWFVGR SNAQGIDLNR NFPDLDRIVY VNEKEGGPNN HLLKNMKKIV DQNTKLAPET KAVIHWIMDI PFVLSANLHG GDLVANYPYD ETRSGSAHEY SSSPDDAIFQ SLARAYSSFN PAMSDPNRPP CRKNDDDSSF VDGTTNGGAW YSVPGGMQDF NYLSSNCFEI TVELSCEKFP PEETLKTYWE DNKNSLISYL EQIHRGVKGF VRDLQGNPIA NATISVEGID HDVTSAKDGD YWRLLIPGNY KLTASAPGYL AITKKVAVPY SPAAGVDFEL ESFSERKEEE KEELMEWWKM MSETLNF