Recombinant humaan CARD18-eiwit

Caspase recruitment domain-containing protein 18, also known as CARD18, is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The association of CARD18 with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18. Recombinant human CARD18 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Caspase recruitment domain-containing protein 18, ICEBERG, pseudo-ICE, uNQ5804

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

12.3 kDa (110aa) confirmed by MALDI-TOF

CARD18, Caspase recruitment domain-containing protein 18, ICEBERG, pseudo-ICE, uNQ5804, ATGP1460-10 µg, ATGP1460-20 µg, ATGP1460-50 µg, ATGP1460-100 µg, ATGP1460-250 µg, ATGP1460-500 µg, ATGP1460-1 mg, ATGP1460-10, ATGP1460-20, ATGP1460-50, ATGP1460-100, ATGP1460-250, ATGP1460-500, ATGP1460-1

Humke E W., et al. (2000) Cell. 103:99-111.; ; Watson R W., et al. (1998) J Immunol. 161:957-962

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 0.1M NaCl

Apoptosis

NP_067546

P57730

Human

MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH