Recombinant humaan carnosinedipeptide 1/CNDP1-eiwit

CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells. Recombinant human CNDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is >3,000 pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.

Beta-Ala-His dipeptidase, CNDP dipeptidase 1, Carnosine dipeptidase 1, Glutamate carboxypeptidase-like protein 2, Serum carnosinase, CN1, CPGL2, Carnosinase 1

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is >3,000pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.

54.9 kDa (489aa)

Beta-Ala-His dipeptidase, Carnosine dipeptidase 1, Carnosine Dipeptidase1, CN1, CNDP dipeptidase 1, CNDP1, CPGL2, Glutamate carboxypeptidase-like protein 2, HST2308, ATGP3503-10 µg, ATGP3503-20 µg, ATGP3503-50 µg, ATGP3503-100 µg, ATGP3503-250 µg, ATGP3503-500 µg, ATGP3503-1 mg, ATGP3503-010, ATGP3503-020, ATGP3503-050, ATGP3503-100, ATGP3503-250, ATGP3503-500, ATGP3503-01M

Kurashige M., et al. (2013) PLoS ONE 8 (1) :E54064.; ; Ahluwalia TS., et al. (2011) Diabetologia 54 (9) :2295-2302.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_116038.4

Q96KN2

Human

Ser27