Recombinant humaan Caseïnekinase 2-alfa 1/CSNK2A1-eiwit

CSNK2A1, also known as casein kinase II subunit alpha, are catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. It regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. CSNK2A1 may act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. Recombinant human CSNK2A1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Casein kinase 2 alpha 1, Casein kinase II subunit alpha, CK II alpha, CK2A1, Cka1, Cka2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.8 kDa (416aa) confirmed by MALDI-TOF

Casein Kinase2 a, Casein kinase II subunit alpha, CK2A1, CKII, ATGP1455-10 µg, ATGP1455-20 µg, ATGP1455-50 µg, ATGP1455-100 µg, ATGP1455-250 µg, ATGP1455-500 µg, ATGP1455-1 mg, ATGP1455-10, ATGP1455-20, ATGP1455-50, ATGP1455-100, ATGP1455-250, ATGP1455-500, ATGP1455-1

Prudent R., et al. (2010) Cancer Res. 70:9865-9874; ; Deloukas P., et al. (2001) Nature. 414:865-871;

Yoo JY, et al. Nuclear hormone receptor corepressor promotes esophageal cancer cell invasion by transcriptional repression of interferon-γ-inducible protein 10 in a casein kinase 2-dependent manner. (Mol Biol Cell. 2012) {https://pubmed.ncbi.nlm.nih.gov/22675025/}; ; Yoo JY, et al. CK2-NCoR signaling cascade promotes prostate tumorigenesis. (Oncotarget. 2013) {https://pubmed.ncbi.nlm.nih.gov/23669876/}; ; Stakkestad Ø, et al. Phosphorylation Modulates Ameloblastin Self-assembly and Ca 2+ Binding. (Front Physiol. 2017) {https://pubmed.ncbi.nlm.nih.gov/28798693/}; ; Ko H, et al. Phosphorylation-dependent stabilization of MZF1 upregulates N-cadherin expression during protein kinase CK2-mediated epithelial-mesenchymal transition. (Oncogenesis. 2018) {https://pubmed.ncbi.nlm.nih.gov/29540671/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF

Cell Cycle

NP_001886

P68400

Human

MSGPV PSRARVYTDV NTHRPREYWD YESHVVEWGN QDDYQLVRKL GRGKYSEVFE AINITNNEKV VVKILKPVKK KKIKREIKIL ENLRGGPNII TLADIVKDPV SRTPALVFEH VNNTDFKQLY QTLTDYDIRF YMYEILKALD YCHSMGIMHR DVKPHNVMID HEHRKLRLID WGLAEFYHPG QEYNVRVASR YFKGPELLVD YQMYDYSLDM WSLGCMLASM IFRKEPFFHG HDNYDQLVRI AKVLGTEDLY DYIDKYNIEL DPRFNDILGR HSRKRWERFV HSENQHLVSP EALDFLDKLL RYDHQSRLTA REAMEHPYFY TVVKDQARMG SSSMPGGSTP VSSANMMSGI SSVPTPSPLG PLAGSPVIAA ANPLGMPVPA AAGAQQ