Recombinant humaan Caspase-3 eiwit

Caspase-3, also known as caspase-3 isoform a, is a member of the cysteine-aspartic acid protease (caspase) family of enzymes. This protein is most abundant in cell lines of lymphocytic origin. Also, it activates caspases 6 and 7 and the protein itself is processed and is activated by caspases 8, 9, and 10. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits that dimerize to form the active enzyme. Members of the caspase family of proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Recombinant Human Caspase-3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.

CASP3, CPP32, CPP32B, SCA-1, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 5,000pmol/min/ug. One unit will liberate 1pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.

29.4 kDa (256aa) ns)

Caspase3, ATGP3874-10 µg, ATGP3874-20 µg, ATGP3874-50 µg, ATGP3874-100 µg, ATGP3874-250 µg, ATGP3874-500 µg, ATGP3874-1 mg, ATGP3874-10, ATGP3874-20, ATGP3874-50, ATGP3874-100, ATGP3874-250, ATGP3874-500, ATGP3874-1

Walsh JG., et al, (2008) Proc. Natl. Scad. Sci. USA 105:12815.; ; Fernandes-Alnemri T., et al, (1994) J. Biol. Chem. 269:30761-30764.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol

Neuroscience

NP_116786

P42574

Human

Ser29