Recombinant humaan Cathepsine E-eiwit

CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. CTSE, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. Recombinant human CTSE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Cathepsin E, CATE, CTSE

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.4 kDa (330aa)

CTSE, Cathepsin E, CATE, ATGP2586-10 µg, ATGP2586-20 µg, ATGP2586-50 µg, ATGP2586-100 µg, ATGP2586-250 µg, ATGP2586-500 µg, ATGP2586-1 mg, ATGP2586-10, ATGP2586-20, ATGP2586-50, ATGP2586-100, ATGP2586-250, ATGP2586-500, ATGP2586-1

Sealy L., et al. (1996) Eur. J. Immunol. 26:1838-1843; ; Cook M., et al. (2001) Eur. J. Biochem. 268:2658-2668;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_683865

P14091

Human

TESCSMD QSAKEPLINY LDMEYFGTIS IGSPPQNFTV IFDTGSSNLW VPSVYCTSPA CKTHSRFQPS QSSTYSQPGQ SFSIQYGTGS LSGIIGADQV SVEGLTVVGQ QFGESVTEPG QTFVDAEFDG ILGLGYPSLA VGGVTPVFDN MMAQNLVDLP MFSVYMSSNP EGGAGSELIF GGYDHSHFSG SLNWVPVTKQ AYWQIALDNM LWSVPTLTSC RMSPSPLTES PIPSAQLPTP YWTSWMECSS AAVAFKDLTS TLQLGPSGSW GMSSFDSFTQ SLTVGITVWD WPQQSPKEGP CVCACLSDRP